N2 - Macrophages are well known for their role in immune responses and tissue homeostasis. Small Methods published by Wiley-VCH GmbH.
T2 - Real-Time Single-Cell Activation Kinetics of Optically Trapped Macrophages Hence, an unprecedented view of the early kinetics of macrophage polarization is provided.", The approach is validated by successfully following the early phase of an oxidative stress response of macrophages upon phorbol 12-myristate 13-acetate (PMA) stimulation, allowing detailed analysis of the initial macrophage response upon a single biochemical stimulus within seconds after its application, thereby eliminating delay times introduced by other techniques during the stimulation procedure. This strategy enables live-cell imaging at high spatiotemporal resolution and omits surface adhesion and cell-cell contact as biophysical stimuli. Our approach, combining optical tweezers, confocal fluorescence microscopy, and microfluidics, allows us to isolate single macrophages and follow their immediate responses to a biochemical stimulus in real-time. However, little is known about the early kinetics of macrophage polarization in response to single biophysical or biochemical stimuli. They can polarize towards various phenotypes in response to biophysical and biochemical stimuli. Hence, an unprecedented view of the early kinetics of macrophage polarization is provided.Ībstract = "Macrophages are well known for their role in immune responses and tissue homeostasis.
Macrophages are well known for their role in immune responses and tissue homeostasis.
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